Description

The PCV 2XR Test Kit is an enzyme immunoassay designed to detect the presence of antibody to PCV in swine serum. A microtitration format has been configured by coating PCV antigen in alternating columns on the plate. Upon incubation of the test sample in the coated well, antibody specific to PCV forms a complex with the coated viral antigens. After washing away unbound material from the wells, an anti-porcine: horseradish peroxidase conjugate is added that binds to any porcine IgG antibody attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, tetramethylbenzidine (TMB), are added to the wells. Stop the reaction by adding stop solution. Within *5 minutes READ the absorbance at **0nm using **0nm as the reference wavelength if available.